منابع مشابه
Gel filtration of sialoglycoproteins.
The role of sialic acid in the gel-filtration behaviour of sialoglycoproteins was investigated by using the separated isoenzymes of purified human liver alpha-L-fucosidase and several other well-known sialic acid-containing glycoproteins (fetuin, alpha1-acid glycoprotein, thyroglobulin and bovine submaxillary mucin). For each glycoprotein studied, gel filtration of its desialylated derivative g...
متن کاملGel-Filtration Chromatography.
Gel-filtration chromatography is a versatile method that permits the effective separation of biological molecules in high yield. This article describes the basis of the method, the selection of suitable operating conditions, and contrasts typical matrix types. Applications of the technique are described, with references to the scientific literature.
متن کاملMolecular - Weight Estimation by Gel - Filtration
Allen, R. J. L. (1940). Biochem. J. 34, 858. Bartlett, P. D., Grimmett, P., Beers, L. & Shelata, S. (1956). J. biol. Chem. 218, 419. Burton, K. (1956). Biochem. J. 62, 315. Caiger, P., Morton, R. K., Jarrett, I. G. & Filsell, 0. H. (1962). Biochem. J. 85, 351. Filsell, 0. H., Jarrett, I. G., Atkinson, M. R., Caiger, P. & Morton, R. K. (1963). Biochem. J. 89, 92. Fishkin, A. F. & Lata, G. F. (19...
متن کاملRefolding proteins by gel filtration chromatography.
We have developed a facile means for the refolding of milligram quantities of purified proteins that employs gel filtration chromatography. We demonstrate by electrophoretic mobility shift and NMR spectroscopy that human ETS-1 protein, bovine ribonuclease A and E. coli integration host factor can be refolded into the native conformation using this technique. We have extended this strategy to th...
متن کاملA novel insight into membrane fouling mechanism regarding gel layer filtration: Flory-Huggins based filtration mechanism
This study linked the chemical potential change to high specific filtration resistance (SFR) of gel layer, and then proposed a novel membrane fouling mechanism regarding gel layer filtration, namely, Flory-Huggins based filtration mechanism. A mathematical model for this mechanism was theoretically deduced. Agar was used as a model polymer for gel formation. Simulation of the mathematical model...
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ژورنال
عنوان ژورنال: SEIBUTSU BUTSURI KAGAKU
سال: 1973
ISSN: 0031-9082,1349-9785
DOI: 10.2198/sbk.17.155